![]() 5/45 forceps (tip dimensions: 0.1 mm × 0.06 mm catalog number: 11251-35) standard serrated student pattern forceps (11.5-cm length catalog number: 91100-12) and straight Iris serrated forceps (7-cm length catalog number: 11064-07). Stainless Steel Surgical Tools: All surgical tools can be obtained from Fine Science Tools (Foster City, CA nonserrated fine scissors (26-mm straight cutting edge catalog number: 14090-11) Vannas spring scissors (2.5-mm straight cutting edge catalog number: 15000-08) Wecker spring scissors (7-mm angled-cutting edge catalog number: 15010-11) 45-degree angled Dumont no. Syringes (1-ml capacity) with 25–30 gauge needles are required for I.P. A nonsteroidal anti-inflammatory drug (NSAID) analgesic (i.e., ketoprofen: 7.5 mg/kg body weight by subcutaneous (S.C.) injection) should be used for preoperative and postoperative analgesia. Note: A federal license is required to purchase Nembutal® which is classified as a Schedule II narcotic by the U.S. Nembutal® sodium solution (sodium pentobarbital solution) 50 mg/ml NDC 0074-3778-05 (50 ml), which is manufactured by Abbot Laboratories (North Chicago, IL), is supplied by ASD Specialty Health Care (Frisco, TX). Stock Nembutal is diluted in 40% propylene glycol and 10% ethanol in distilled water. If this coordination is not possible, however, host mice (whose mammary fat pad was cleared at 3 weeks of age) can be used at a later time point when donor epithelium is available.Īnesthetic and Analgesic: Nembutal® (35–50 mg/kg body weight by intraperitoneal (I.P.) injection) is a suitable anesthetic for young mice used in this surgical procedure. To avoid multiple surgeries, clearing of the host mammary fat pad and transplantation of donor epithelium is usually performed on the same day. The SCID® Beige mouse (strain code: 250) is supplied by Charles River Laboratories (Wilmington, MA).ĭonor Mice: The appropriate mammary epithelial cells or tissue fragments isolated from donor mice are used for transplantation into the cleared fat pad of host mice. If establishment of an SCID® Beige mouse colony is not feasible, pregnant mice can be ordered to provide litters from which host mice can be obtained. We routinely use immunocompromised Fox Chase SCID® Beige mice with a white/albino coat for this purpose. Host Mice: To prevent rejection of transplanted donor epithelium, a syngeneic (i.e., isogenic to the donor) or an immunocompromised strain of mouse (3 weeks of age) must be used as the host. Paula Amato, in The Guide to Investigation of Mouse Pregnancy, 2014 Mice and Materials
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